Coding

Part:BBa_K4033004:Design

Designed by: Chenxi Wang   Group: iGEM21_BHSF   (2021-08-24)


DnrF for coding


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 508
    Illegal NotI site found at 1219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1074
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 508
    Illegal AgeI site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DnrF has substrate specificity, so host optimization is needed.


Source

Streptomyces peucetius

References